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| Catalog Number | orb775960 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin Endoperoxide Synthase 2(PTGS2). |
| Reactivity | Human |
| Concentration | 20 ng/mL |
| UniProt ID | P35354 |
| Tested applications | ELISA |
| Application notes | standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PTGS2/COX-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PTGS2/COX-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PTGS2/COX-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PTGS2/COX-2 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 0.32-20 ng/mL |
| Sensitivity | 0.125 ng/mL |
| Sample Types | serum, plasma, tissue homogenates and other biological fluids |
| Alternative names | PGG/HS ELISA Kit, PGHS2 ELISA Kit, PHS2 ELISA Kit, Read more... |
| Note | For research use only |
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- Item 1 of 1
Human Prostaglandin Endoperoxide Synthase 2 (PTGS2/COX-2) ELISA Kit [orb1807242]
Human
0.31-20 ng/mL
0.16 ng/mL
96 T, 48 T
