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Human Proopiomelanocortin (POMC) ELISA Kit

Catalog Number: orb775739

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SizePriceQuantity
48 T$ 320.00
96 T$ 450.00
48 T Enquire
96 T Enquire
DispatchUsually dispatched within 5-10 working days
Catalog Numberorb775739
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Proopiomelanocortin(POMC) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Proopiomelanocortin(POMC).
ReactivityHuman
Concentration10000 pg/mL
UniProt IDP01189
Tested applicationsELISA
Application notesstandard: 10000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human POMC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human POMC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human POMC in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeCompetitive
Assay Time2.5h
Range156.25-10000 pg/mL
Sensitivity44 pg/mL
Sample TypesSerum, plasma and other biological fluids
Alternative namesPOC ELISA Kit, NPP ELISA Kit, ProOMC ELISA Kit, AC
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Research AreaMetabolism Research
NoteFor research use only
Expiration DatePlease enquire.
Human Proopiomelanocortin (POMC) ELISA Kit