Human Proliferation Associated Protein 2G4 (PA2G4) ELISA Kit

SKU: orb779987

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proliferation Associated Protein 2G4(PA2G4).

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PA2G4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PA2G4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PA2G4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PA2G4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.112 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

EBP1 ELISA Kit, HG4-1 ELISA Kit, p38-2G4 ELISA Kit, Cell cycle protein p38-2G4 homolog ELISA Kit, ErbB3-binding protein 1 ELISA Kit

Quick Database Links

UniProt

Q9UQ80