Human Prolactin Induced Protein (PIP) ELISA Kit

SKU: orb780130

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolactin Induced Protein(PIP).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.63-40 ng/mL
• Sensitivity: 0.261 ng/mL
• Concentration: 40 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

GCDFP15 ELISA Kit, GPIP4 ELISA Kit, BRST2 ELISA Kit, SABP ELISA Kit, gp17 ELISA Kit, Gross cystic disease fluid protein 15 ELISA Kit, Prolactin-induced protein ELISA Kit, Secretory actin-binding protein ELISA Kit

Quick Database Links

UniProt

P12273