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Human Progranulin (PGRN) ELISA Kit

Human Progranulin (PGRN) ELISA Kit

Catalog Number: orb2667373

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2667373
CategoryAssays and Kits
DescriptionHuman Progranulin (PGRN) ELISA Kit
Range1.56-100 ng/mL
Assay Time3.5h
UniProt IDP28799
Sensitivity0.51 ng/mL
Alternative namesPGRN; Acrogranin; GEP; GP88; GRN; PCDGF; PEPI; Pro
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NoteFor research use only
Application notesstandard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PGRN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PGRN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PGRN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGRN in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesSerum, plasma and other biological fluids.
Expiration DatePlease enquire.
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