Human Progestagen Associated Endometrial Protein (PAEP) ELISA Kit

SKU: orb777395

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Progestagen Associated Endometrial Protein(PAEP).

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 300 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PAEP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 4.69-300 ng/mL
• Sensitivity: 2.1 ng/mL
• Concentration: 300 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

GD ELISA Kit, GdA ELISA Kit, GdF ELISA Kit, GdS ELISA Kit, PAEG ELISA Kit, PEP ELISA Kit, PP14 ELISA Kit, Glycodelin ELISA Kit, Placental Protein 14 ELISA Kit, Alpha Uterine Protein ELISA Kit, Pregnancy-Associated Endometrial Alpha-2-Globulin ELISA Kit, Progesterone-associated ELISA Kit

Quick Database Links

UniProt

P09466