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| Catalog Number | orb866983 |
|---|---|
| Category | Assays and Kits |
| Description | Sandwich Human Pro-ADM ELISA Kit |
| Reactivity | Human |
| Concentration | 20 ng/mL |
| UniProt ID | P35318 |
| Tested applications | ELISA |
| Application notes | standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Pro-ADM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Pro-ADM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Pro-ADM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Pro-ADM in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 0.32-20 ng/mL |
| Sensitivity | 0.109 ng/mL |
| Sample Types | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | Proadrenomedullin ELISA Kit |
| Note | For research use only |
Human Pro-ADM ELISA Kit [orb407064]
Human
0.312 pmol/L-20 pmol/L
0.078 pmol/L
24 T, 10 x 96 T, 48 T, 96 T, 5 x 96 T- Item 1 of 1
Human Proadrenomedullin (Pro-ADM) ELISA Kit [orb1807244]
Human
0.78-50 pmol/mL
0.114 pmol/mL
96 T, 48 T
