Human pro-Beta NGF Protein

• Catalog Number: orb1471730
• Category: Proteins
• Description: Recombinant Human pro-Nerve Growth Factor is produced by our E.coli expression system and the target gene encoding Glu19-Ala241 is expressed.
• Reactivity: Canine
• Tag: C-Mouse Fc tag
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• MW: 25 KDa
• Target: pro-Beta
• UniProt ID: P01138
• Source: Mammalian
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Buffer/Preservatives: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl,500mM NaCl,5%Trehalose,5%Mannitol,0.01%tween80,1mM EDTA,pH8.0.
• Alternative names: Beta-Nerve Growth Factor;Beta-NGF;NGF;NGFB;-NGF;pr
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Canine PD-1 Protein, mFc Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/mL (100 μL/well) Canine PD-1 Protein, mFc Tag (orb2321234) can bind Anti-Canine PD-1 (INTERVET 4F12) mAb (orb2321976) in a linear range of 0.64–80 ng/ml.

ELISA plate pre-coated by 2 μg/mL (100 μL/well) Canine PD-1 Protein, mFc Tag (orb2321234) can bind Canine PD-L1 Protein, hFc Tag (orb2321231) in a linear range of 16–2000 ng/ml.

Flow cytometry analysis with 1 μg/mL Canine PD-1 Protein, mFc Tag (orb2321234) on Expi293 cells transfected with Canine PD-L1 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).