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| Catalog Number | orb1146836 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pro Atrial Natriuretic Peptide(Pro-ANP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pro Atrial Natriuretic Peptide(Pro-ANP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pro Atrial Natriuretic Peptide(Pro-ANP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pro Atrial Natriuretic Peptide(Pro-ANP) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Concentration | 10 ng/mL |
| UniProt ID | P01160 |
| Application notes | standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Pro-ANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Pro-ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Pro-ANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Pro-ANP in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 0.16-10 ng/mL |
| Sensitivity | 0.058 ng/mL |
| Sample Types | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | NT-proANP, Pro Atrial Natriuretic Peptide |
| Note | For research use only |
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Human CORIN ELISA Kit [orb405699]
Human
0.625 ng/mL-40 ng/mL
0.156 ng/mL
48 T, 96 T, 10 x 96 T, 5 x 96 T, 24 THuman Pro-ANP ELISA Kit [orb406932]
Human
62.5 pg/mL-4000 pg/mL
15.6 pg/mL
48 T, 96 T, 10 x 96 T, 5 x 96 T, 24 T- Item 1 of 1
- Item 1 of 1
