Human Pregnancy Specific Beta-1-Glycoprotein 2 (PSG2) ELISA Kit

SKU: orb778620

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pregnancy Specific Beta-1-Glycoprotein 2(PSG2).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSG2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PSG2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PSG2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSG2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, breast milk, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.79-50 ng/mL
• Sensitivity: 0.34 ng/mL
• Concentration: 50 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CEA ELISA Kit, PSG1 ELISA Kit, PSBG2 ELISA Kit, PSGGB ELISA Kit, Carcinoembryonic Antigen SG8 ELISA Kit, Pregnancy-specific glycoprotein 2 ELISA Kit, Pregnancy-specific beta-1 glycoprotein E ELISA Kit

Quick Database Links

UniProt

P11465