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Human Pleiotrophin (PTN) ELISA Kit

Catalog Number: orb1949115

DispatchUsually dispatched within 5-10 working days
$ 280.00
Catalog Numberorb1949115
CategoryAssays and Kits
DescriptionHuman Pleiotrophin (PTN) ELISA Kit
ReactivityHuman
Range31.25-2000pg/mL
Assay Time3.5H
TargetPTN
UniProt IDP21246
Sensitivity18.75pg/mL
Alternative namesHARP, HBGF8, HBNF, NEGF1
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NoteFor research use only
Application notesThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PTN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PTN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PTN. You can calculate the concentration of Human PTN in the samples by comparing the OD of the samples to the standard curve.
Sample TypesSerum, Plasma, Tissue homogenate and Other biological samples
Expiration DatePlease enquire.
Human Pleiotrophin (PTN) ELISA Kit

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