Human Placental Thrombin Inhibitor (PTI) ELISA Kit

SKU: orb778746

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Placental Thrombin Inhibitor(PTI).

Research Area

Hematology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PTI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PTI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PTI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PTI in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.057 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

SERPINB6 ELISA Kit, CAP ELISA Kit, PI6 ELISA Kit, SPI3 ELISA Kit, Serpin Peptidase Inhibitor Clade B Member 6 ELISA Kit, Cytoplasmic antiproteinase ELISA Kit, Peptidase inhibitor 6 ELISA Kit

Quick Database Links

UniProt

P35237