Human Phospholipase A2 Receptor 1 (PLA2R1) ELISA Kit

SKU: orb777422

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2 Receptor 1(PLA2R1).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLA2R1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PLA2R1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PLA2R1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PLA2R1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.058 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CLEC13C ELISA Kit, PLA2G1R ELISA Kit, PLA2IR ELISA Kit, PLA2R ELISA Kit, 180 kDa secretory phospholipase A2 receptor ELISA Kit, C-type lectin domain family 13 member C ELISA Kit, Soluble secretory phospholipase A2 receptor ELISA Kit

Quick Database Links

UniProt

Q13018