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| Catalog Number | orb1949283 |
|---|---|
| Category | Assays and Kits |
| Description | Human Phosphoinositide-3-Kinase Catalytic Delta Polypeptide (PIK3Cσ) ELISA Kit |
| Target | PIK3Cσ |
| Reactivity | Human |
| UniProt ID | O00329 |
| Application notes | This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PIK3Cσ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PIK3Cσ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PIK3Cσ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PIK3Cσ. You can calculate the concentration of Human PIK3Cσ in the samples by comparing the OD of the samples to the standard curve. |
| Assay Time | 3.5H |
| Range | 0.32-20ng/mL |
| Sensitivity | 0.19ng/mL |
| Sample Types | Serum, Plasma, Tissue homogenate and Other biological samples |
| Alternative names | PIK3CD, APDS, IMD14, P11DELTA, PI3K, p11D, phospha Read more... |
| Note | For research use only |
