Human Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a) ELISA Kit

• Catalog Number: orb1088144
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide(PIK3C2a). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide(PIK3C2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide(PIK3C2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide(PIK3C2a) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Concentration: 10 ng/mL
• UniProt ID: O00443
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIK3C2a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIK3C2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIK3C2a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIK3C2a in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.059 ng/mL
• Sample Types: Tissue homogenates and other biological fluids.
• Alternative names: PI3K2a, CPK, PI3-K-C2(Alpha), PI3-K-C2A, PI3K2-a,
• Note: For research use only