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| Catalog Number | orb1736550 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human p-AKT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human p-AKT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human p-AKT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human p-AKT in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Concentration | 10000 pg/mL |
| Application notes | standard: 10000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human p-AKT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human p-AKT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human p-AKT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human p-AKT in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 3.5h |
| Range | 156.25-10000 pg/mL |
| Sensitivity | 52.5 pg/mL |
| Sample Types | tissue homogenates, cell lysates and other biological fluids |
| Alternative names | p-AKT |
| Note | For research use only |
