Human Phospho AKT S473 (p-AKT) ELISA Kit

• Catalog Number: orb1736550
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human p-AKT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human p-AKT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human p-AKT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human p-AKT in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Range: 156.25-10000 pg/mL
• Sensitivity: 51.3 pg/mL
• Alternative names: p-AKT
• Note: For research use only
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Expiration Date: Please enquire.