Human Phosphatidylinositol-glycan biosynthesis Class F Protein (PIGF) ELISA Kit

SKU: orb782828

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PIGF(Phosphatidylinositol-glycan biosynthesis class F protein) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PIGF(Phosphatidylinositol-glycan biosynthesis class F protein).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PIGF protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIGF in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 15.6-1000 ng/mL
• Sensitivity: 6.2 ng/mL
• Concentration: 1000 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

PHOSPHATIDYLINOSITOL GLYCAN ELISA Kit, Phosphatidylinositol glycan biosynthesis class F protein ELISA Kit, Phosphatidylinositol-glycan biosynthesis class F protein ELISA Kit, PIG F ELISA Kit, PIG-F ELISA Kit, PIGF ELISA Kit, PIGF_HUMAN ELISA Kit, PIGF1 ELISA Kit

Quick Database Links

UniProt

Q07326