You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb782693 |
---|---|
Category | Assays and Kits |
Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PNX-20(Phoenixin-20). |
Tested applications | ELISA |
Reactivity | Human |
Range | 15.63-1000 pg/mL |
Assay Time | 3.5h |
Concentration | 1000 pg/mL |
UniProt ID | Q8N5G0 |
Sensitivity | 7.3 pg/mL |
Alternative names | Small integral membrane protein ELISA Kit, Mitocho Read more... |
Note | For research use only |
Application notes | standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PNX-20. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PNX-20. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PNX-20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PNX-20 in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | Serum, plasma and other biological fluids. |
Expiration Date | Please enquire. |