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Human Phoenixin-20 (PNX-20) ELISA Kit

Catalog Number: orb782693

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb782693
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PNX-20(Phoenixin-20).
Tested applicationsELISA
ReactivityHuman
Range15.63-1000 pg/mL
Assay Time3.5h
Concentration1000 pg/mL
UniProt IDQ8N5G0
Sensitivity7.3 pg/mL
Alternative namesSmall integral membrane protein ELISA Kit, Mitocho
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NoteFor research use only
Application notesstandard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PNX-20. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PNX-20. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PNX-20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PNX-20 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesSerum, plasma and other biological fluids.
Expiration DatePlease enquire.
Human Phoenixin-20 (PNX-20) ELISA Kit

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