Human Phenylalanine Hydroxylase (PAH) ELISA Kit

SKU: orb777711

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phenylalanine Hydroxylase(PAH).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PAH in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.55 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

PKU ELISA Kit, PKU1 ELISA Kit, Phe-4-monooxygenase ELISA Kit

Quick Database Links

UniProt

P00439