Human Phenol Sulfotransferase (PST) ELISA Kit

SKU: orb777110

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phenol Sulfotransferase(PST).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PST in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.058 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

SULT1A1 ELISA Kit, HAST1/HAST2 ELISA Kit, ST1A3 ELISA Kit, STP1 ELISA Kit, TSPST1 ELISA Kit, Sulfotransferase 1A1 ELISA Kit, Aryl sulfotransferase 1 ELISA Kit, Phenol sulfotransferase 1 ELISA Kit, Thermostable phenol sulfotransferase ELISA Kit

Quick Database Links

UniProt

P50225