Human Peroxisome Proliferator Activated Receptor Delta (PPARd) ELISA Kit

SKU: orb776626

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Delta(PPARd).

Research Area

Cancer Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PPARd. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PPARd. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PPARd, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PPARd in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.117 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

FAAR ELISA Kit, NR1C2 ELISA Kit, NUC1 ELISA Kit, NUCI ELISA Kit, PPARB ELISA Kit, PPARδ/B ELISA Kit, Nuclear Receptor Subfamily 1,Group C,Member 2 ELISA Kit, Nuclear hormone receptor 1 ELISA Kit, Peroxisome proliferator-activated receptor beta ELISA Kit

Quick Database Links

UniProt

Q03181