Human Peroxisomal Biogenesis Factor 2 (PEX2) ELISA Kit

SKU: orb779136

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisomal Biogenesis Factor 2(PEX2).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PEX2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PEX2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PEX2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PEX2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.058 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

PXMP3 ELISA Kit, PAF1 ELISA Kit, PMP35 ELISA Kit, PMP3 ELISA Kit, RNF72 ELISA Kit, Peroxin 2 ELISA Kit, Peroxisome Assembly Factor 1 ELISA Kit, Peroxisomal Membrane Protein 35kDa ELISA Kit, Peroxisomal Membrane Protein 3 ELISA Kit

Quick Database Links

UniProt

P28328