Human Peripheral Myelin Protein 22 (PMP22) ELISA Kit

SKU: orb778335

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peripheral Myelin Protein 22(PMP22).

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PMP22. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PMP22. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PMP22, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PMP22 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.65 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

DSS ELISA Kit, CMT1A ELISA Kit, CMT1E ELISA Kit, GAS3 ELISA Kit, HMSNIA ELISA Kit, HNPP ELISA Kit, Sp110 ELISA Kit, Growth arrest-specific protein 3 ELISA Kit

Quick Database Links

UniProt

Q01453