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Human Peptide YY (PYY) ELISA Kit
Catalog Number: orb775536
| Catalog Number | orb775536 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Peptide YY(PYY) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Peptide YY(PYY). |
| Reactivity | Human |
| Concentration | 1000 pg/mL |
| UniProt ID | P10082 |
| Tested applications | ELISA |
| Application notes | standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PYY protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PYY. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PYY in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Competitive |
| Assay Time | 2.5h |
| Range | 15.63-1000 pg/mL |
| Sensitivity | 4.6 pg/mL |
| Sample Types | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | PYY1 ELISA Kit, Peptide tyrosine tyrosine ELISA Ki Read more... |
| Research Area | Gastroenterology & Hepatology, Neuroscience |
| Note | For research use only |
| Expiration Date | Please enquire. |
- Item 1 of 1
- Item 1 of 1
Human PYY ELISA Kit [orb406221]
Human
62.5 pg/mL-4000 pg/mL
15.6 pg/mL
96 T, 10 x 96 T, 5 x 96 T, 48 T, 24 T- Item 1 of 1
