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Catalog Number | orb570289 |
---|---|
Category | Proteins |
Description | Human PDGF R alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Ala 528 (Accession # P16234-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 58.4 kDa |
Target | PDGF R alpha |
UniProt ID | P16234 |
Protein Sequence | NP_006197.1 |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Gln 24 - Ala 528 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_006197.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 58.4 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
98.00% | |
59.2 kDa (predicted). Due to glycosylation, the protein migrates to 70-83 kDa based on Tris-Bis PAGE result. |
98.00% | |
59.2 kDa (predicted). Due to glycosylation, the protein migrates to 70-83 kDa based on Tris-Bis PAGE result. |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Biotinylated Recombinant Human PDGF R alpha / PDGFRA Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Met1-Glu524) of Human PDGF R alpha / PDGFRA fused with His Tag and Avi tag at the C-terminal. |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Recombinant Human PDGF R alpha / PDGFRA Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Met1-Glu524) of Human PDGF R alpha / PDGFRA fused with His Tag and Avi tag at the C-terminal. |