Human PCDHb16(Protocadherin Beta 16) Microsample ELISA Kit

SKU: orb2998465

Description

Human PCDHb16(Protocadherin Beta 16) Microsample ELISA Kit

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PCDHb16. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PCDHb16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PCDHb16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PCDHb16 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.054 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PCDHB8a; PCDH3X; PCDH-BETA16; ME1; Cadherin ME1; Protocadherin-3x; PCDHbeta 16

Quick Database Links

UniProt

Q9NRJ7