Human Pancreatic Polypeptide (PP) ELISA Kit

• Catalog Number: orb779336
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pancreatic Polypeptide(PP).
• Tested applications: ELISA
• Reactivity: Human
• Range: 0.32-20 ng/mL
• Assay Time: 3.5h
• Concentration: 20 ng/mL
• UniProt ID: P01298
• Sensitivity: 0.128 ng/mL
• Alternative names: PPY , PNPPancreatic prohormone , Pancreatic polype
• Note: For research use only
• Application notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PP in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Expiration Date: Please enquire.