Human Palate/Lung and Nasal Epithelium Associated Protein (PLUNC) ELISA Kit

SKU: orb778153

Description

The Human Palate/Lung and Nasal Epithelium Associated Protein (PLUNC) ELISA Kit uses a sandwich enzyme immunoassay to quantify Palate/Lung and Nasal Epithelium Associated Protein (PLUNC) in human serum, plasma, tissue homogenates, cell lysates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.054 ng/mL.

Research Area

Immunology & Inflammation; Respiratory Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLUNC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PLUNC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PLUNC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PLUNC in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.054 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

BPIFA1 ELISA Kit, SPURT ELISA Kit, NASG ELISA Kit, LUNX ELISA Kit, SPLUNC1 ELISA Kit, Secretory Protein In Upper Respiratory Tracts ELISA Kit, BPI Fold Containing Family A, Member 1 ELISA Kit, Nasopharyngeal carcinoma-related ELISA Kit

Yue Guo, Lin-Na Guo, Jun-Fei Zhu, Chen-Yi Tang, Yun-Zhi Feng, Hou-De Zhou Associations of Salivary BPIFA1 Protein in Chronic Periodontitis Patients with Type 2 Diabetes Mellitus Int J Endocrinol, 2017, 1 (2017)

PubMed: 29109737

Applications

ELISA

Reactivity

Human