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Catalog Number | orb633330 |
---|---|
Category | Proteins |
Description | Recombinant human PAI-1 protein |
Tag | N-terminal His Tag |
Reactivity | Human |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH 7.4 |
Purity | Greater than 95% by SDS-PAGE gel analyses |
UniProt ID | P05121 |
MW | 42.7 KDa |
Tested applications | ELISA, WB |
Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
Source | E.Coli |
Expression Region | 24-402aa |
Storage | -20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution |
Alternative names | Endothelial plasminogen activator inhibitor, Nexin Read more... |
Note | For research use only |
Greater than 90% as determined by SDS-PAGE. | |
46.7 kDa | |
E.coli |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 43.6 kDa after removal of the signal peptide. The apparent molecular mass of PAI1-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
> 90% as determined by SDS-PAGE. | |
45.27 kDa |
> 97%, determined by SDS-PAGE | |
This protein contains the human SerpinE1 precursor (NP_0005931) (Met 1-Pro 402) was expressed with a C-terminal polyhistidine tag and expressed from HEK293 Cells. |