Human p53 Upregulated Modulator of Apoptosis (PUMA) ELISA Kit

• Catalog Number: orb776538
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to p53 Upregulated Modulator Of Apoptosis(PUMA).
• Tested applications: ELISA
• Reactivity: Human
• Range: 0.16-10 ng/mL
• Assay Time: 3.5h
• Concentration: 10 ng/mL
• UniProt ID: Q9BXH1
• Sensitivity: 0.058 ng/mL
• Alternative names: JFY1 ELISA Kit, BBC3 ELISA Kit, BCL2 Binding Compo
• Note: For research use only
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PUMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PUMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PUMA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PUMA in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Expiration Date: Please enquire.