Human p-AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit

• Catalog Number: orb2999118
• Category: Assays and Kits
• Description: Human p-AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit
• Application notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human p-AMPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human p-AMPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human p-AMPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human p-AMPK in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.121 ng/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: p-PRKAA1; p-AMPK; p-AMPKa1; p-AMPK alpha 1
• Note: For research use only
• Expiration Date: Please enquire.