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Human Osteoglycin (OGN) ELISA Kit

Human Osteoglycin (OGN) ELISA Kit

Catalog Number: orb2667770

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2667770
CategoryAssays and Kits
DescriptionHuman Osteoglycin (OGN) ELISA Kit
UniProt IDP20774
Application notesstandard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OGN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OGN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OGN in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeSandwich
Assay Time3.5h
Range0.32-20 ng/mL
Sensitivity0.13 ng/mL
Sample TypesSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Alternative namesOIF; SLRR3A; Mimecan Proteoglycan; Osteoinductive
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NoteFor research use only
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