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Catalog Number | orb392224 |
---|---|
Category | Proteins |
Description | Recombinant human OSM protein is produced by our E. coli expression system and the target gene encoding Ala26-Arg221 is expressed with a 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | OSM |
Entrez | 5008 |
UniProt ID | P13725 |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MAAIGSCSKE YRVLLGQLQK QTDLMQDTSR LLDPYIRIQG LDVPKLREHC RERPGAFPSE ETLRGLGRRG FLQTLNATLG CVLHRLADLE QRLPKAQDLE RSGLNIEDLE KLQMARPNIL GLRNNIYCMA QLLDNSDTAE PTKAGRGASQ PPTPTPASDA FQRKLEGCRF LHGYHRFMHS VGRVFSKWGE SPNRSRR |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Alternative names | Oncostatin-M; OSM Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 97% as determined by SDS-PAGE and HPLC. | |
22.0 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
23.7 kDa | |
E.Coli |
> 95% as determined by SDS-PAGE and HPLC. | |
25.8 kDa | |
E.Coli |
Unconjugated | |
95% | |
25.8 kDa | |
Human Oncostatin M Protein, premium grade (orb257725) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Arg 252 (Accession # NP_065391.1). |
Unconjugated | |
95% | |
39.6 kDa | |
Human IL-11 R alpha, His Tag (orb1149284) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Ala 370 (Accession # Q14626-1). |
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