Human Orexin (OX) ELISA Kit

SKU: orb778585

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Orexin(OX) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Orexin(OX).

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human OX protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OX in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 15.63-1000 pg/mL
• Sensitivity: 4.6 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

HCRT ELISA Kit, PPOX ELISA Kit, Orexin A ELISA Kit, Hypocretin 1 ELISA Kit, Orexin B ELISA Kit, Hypocretin 2 ELISA Kit, Preproorexin ELISA Kit, Hypocretin Neuropeptide Precursor ELISA Kit

Quick Database Links

UniProt

O43612