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Human Opiorphin (OPI) ELISA Kit

Catalog Number: orb1291278

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb1291278
CategoryAssays and Kits
DescriptionHuman OPI (Opiorphin) ELISA Kit
ReactivityHuman
Concentration10 ng/mL
UniProt IDQ99935
Tested applicationsELISA
Application notesstandard: 10 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human OPI protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OPI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OPI in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time2.5h
Range0.16-10 ng/mL
Sensitivity0.05 ng/mL
Sample Typestissue homogenates, cell lysates, cell culture supernates and other biological fluids
Alternative namesOpiorphin
NoteFor research use only
Human Opiorphin (OPI) ELISA Kit

  • Human Opi ELISA Kit [orb563347]

    Human

    0.156-10ng/ml

    0.094ng/ml

    96 T, 48 T