Human Nuclear Transcription Factor Y Gamma (NFYC) ELISA Kit

SKU: orb777827

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Transcription Factor Y Gamma(NFYC).

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NFYC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NFYC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NFYC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NFYC in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.109 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CBF-C ELISA Kit, NFYg ELISA Kit, CBFC ELISA Kit, H1TF2A ELISA Kit, HAP5 ELISA Kit, HSM ELISA Kit, NF-YC ELISA Kit, hCBF-C ELISA Kit, CAAT box DNA-binding protein subunit C ELISA Kit, Nuclear transcription factor Y subunit C ELISA Kit, Transactivator HSM-1/2 ELISA Kit

Quick Database Links

UniProt

Q13952