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Human NUCB2 protein

SKU: orb707016

Description

Recombinant Human Nucleobindin-2 Protein is produced by E.coli expression system and the target gene encoding Val25-Leu106 is expressed.

Research Area

Cell Biology

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
TargetNUCB2
ReactivityHuman
EndotoxinsLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
PurityGreater than 95% as determined by reducing SDS-PAGE.
Protein SequenceVPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized
Buffer/Preservatives0.2 μm filtered solution of 10mM Sodium Phosphate, pH 6.5.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

NEFA; Nucleobindin-2; DNA-binding protein NEFA; Gastric cancer antigen Zg4; Prepronesfatin

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human NUCB2 protein (orb707016)

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Available Sizes

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10 μg
$ 380.00
50 μg
$ 1,000.00
500 μg
$ 4,850.00
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