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| Catalog Number | orb427415 |
|---|---|
| Category | Proteins |
| Description | Recombinant of human NT 4 protein |
| Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Buffer/Preservatives | Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM phosphate buffer pH-7.4 and 150mM NaCl. |
| Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Protein Sequence | GVSETAPASR RGELAVCDAV SGWVTDRRTA VDLRGREVEV LGEVPAAGGS PLRQYFFETR CKADNAEEGG PGAGGGGCRG VDRRHWVSEC KAKQSYVRAL TADAQGRVGW RWIRIDTACV CTLLSRTGRA |
| Application notes | Cytokines And Growth Factors |
| Source | Escherichia Coli |
| Biological Activity | Determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 20-50 ng/ml. |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized NT-4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage | Stability: Lyophilized NT-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NT-4 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
| Alternative names | NT4, NT5, NTF5, NT-4/5, NTF4, Neurotrophin-4, Neut Read more... |
| Note | For research use only |
> 97% as determined by SDS-PAGE and HPLC. | |
14.1 kDa | |
E.Coli |
Greater than 90% as determined by SDS-PAGE. | |
17.9 kDa | |
E.coli |
>90% as determined by SDS-PAGE. | |
31.85 kDa |
>90% as determined by SDS-PAGE. | |
16.23 kDa |
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