Recombinant Human NT-3 Protein

SKU: orb3002430

Description

Recombinant Human Neurotrophin-3 is produced by our E.coli expression system and the target gene encoding Tyr139-Thr257 is expressed.

Key Properties

• Expression System: E.coli
• Biological Activity: ELISA: Immobilized Human TrkB (C-6His)(Cat. No.:orb2995029) at 5 μg/mL (100 μL/well) can bind Human NT-3(Cat. No.:orb3002430)*: Biotinylated by NHS-biotin prior to testing. The EC50 of Human NT-3(Cat. No.:orb3002430)* is 15 ng/mL.(Regularly tested).
• Tag: Tag free
• Molecular Weight: Predicted: 13.6 KDa. Observed: 14 KDa, reducing conditions
• Expression Region: Tyr139-Thr257
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)
• Endotoxins: < 0.01 EU/µg as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Neurotrophin-3; NT-3; HDNF; Nerve Growth Factor 2; NGF-2; Neurotrophic Factor; NTF3

Quick Database Links

UniProt

P20783