Human Nephrin (NPHN) ELISA Kit

SKU: orb775907

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nephrin(NPHN).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NPHN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NPHN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NPHN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NPHN in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.056 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

NPHS1 ELISA Kit, CNF ELISA Kit, Nephrosis 1 ELISA Kit, Congenital Finnish Type ELISA Kit, Renal glomerulus-specific cell adhesion receptor ELISA Kit

Quick Database Links

UniProt

O60500

References

C Li, JKC Ng, GCK Chan, WWS Fung, KM Chow, CC Szeto Preservation of Urinary Podocyte Markers in Diabetic Kidney Disease by SGLT2 Inhibitor Therapy Kidney Diseases,, (2025)

PubMed:

Applications

ELISA

Reactivity

Human