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Catalog Number | orb257704 |
---|---|
Category | Proteins |
Description | Human NKp46, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asn 254 (Accession # AAH64806). |
Reactivity | Human |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 53.0 kDa |
Target | NKp46, NCR1, CD335 |
UniProt ID | O76036 |
Source | Human NKp46, Fc Tag (orb257704) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asn 254 (Accession # AAH64806). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human NKp46, Fc Tag, premium grade at 1 μg/mL (100 μL/well) can bind Anti-NKP46 Antibody, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested). |
Expression Region | Gln 22 - Asn 254 |
Endotoxins | 0.01 EU per μg |
NCBI | AAH64806 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 10 mM NaCl, pH7.5 |
Alternative names | NCR1,LY94,CD335,NK-p46,hNKp46 Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 53.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human NKp46 protein
Immobilized Human NKp46, Fc Tag, premium grade (Cat. No. orb257704) at 1 μg/mL (100 μL/well) can bind Anti-NKP46 Antibody, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested).
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 52.5 kDa after removal of the signal peptide. The apparent molecular mass of NCR1-hFc is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
Unconjugated | |
90% | |
36.4 kDa | |
Human NKp46 Protein, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Glu 34 - Thr 118 (Accession # O76036-1). |
Unconjugated | |
35.8 kDa | |
Human NKp46 Protein, Fc Tag is expressed from human 293 cells (HEK293). It contains AA His 129 - Thr 211 (Accession # O76036-1). |