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Catalog Number | orb689425 |
---|---|
Category | Proteins |
Description | Recombinant human NKp30 protein with C-terminal human Fc |
Reactivity | Human |
Tag | C-Human Fc Tag |
Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
MW | The protein has a predicted molecular mass of 39.0 kDa after removal of the signal peptide.The apparent molecular mass of NKp30-hFc is approximately 45-60 kDa due to glycosylation. |
Target | NKp30 |
UniProt ID | O14931 |
Source | Mammalian |
Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Buffer/Preservatives | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
Alternative names | NCR3;CD337;NKp30;1C7;LY117;MALS Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Human B7-1 Protein, hFc Tag on SDS-PAGE under reducing condition.
ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-1, hFc tagged protein (orb689457) can bind Human CTLA4, mFc-His tagged protein orb689378 in a linear range of 0.048-2.094 ng/ml.
ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-1, hFc tagged protein (orb689457) can bind Human CD28, mFc-His tagged protein orb689375 in a linear range of 125-4000 ng/ml.
HEK293 cell line transfected with irrelevant protein (B) and human CD28 (A) were surface stained with Human B7-1, hFc tagged protein (orb689457) 1 μg/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.
98.00% | |
53.2 kDa (predicted). Due to glycosylation, the protein migrates to 68-70 kDa based on Tris-Bis PAGE result. |