Human NKp30 Protein, hFc Tag

SKU: orb689425

Description

Recombinant human NKp30 protein with C-terminal human Fc

Key Properties

• Source: Mammalian
• Expression System: HEK293
• Target: NKp30
• Reactivity: Human
• Tag: C-Human Fc Tag
• Molecular Weight: The protein has a predicted molecular mass of 39.0 kDa after removal of the signal peptide.The apparent molecular mass of NKp30-hFc is approximately 45-60 kDa due to glycosylation.
• Expression Region: NKp30(Leu19-Gly135) hFc(Glu99-Ala330)
• Purification: Purified from cell culture supernatant by affinity chromatography
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Buffer/Preservatives: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Reconstitution: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Disclaimer: For research use only

Alternative Names

NCR3;CD337;NKp30;1C7;LY117;MALS

Human B7-1 Protein, hFc Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-1, hFc tagged protein (orb689457) can bind Human CTLA4, mFc-His tagged protein orb689378 in a linear range of 0.048-2.094 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-1, hFc tagged protein (orb689457) can bind Human CD28, mFc-His tagged protein orb689375 in a linear range of 125-4000 ng/ml.

HEK293 cell line transfected with irrelevant protein (B) and human CD28 (A) were surface stained with Human B7-1, hFc tagged protein (orb689457) 1 μg/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.

Quick Database Links

Gene Symbol

NKp30

UniProt

O14931