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| Catalog Number | orb778377 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nischarin(NISCH). |
| Reactivity | Human |
| Concentration | 10 ng/mL |
| UniProt ID | Q9Y2I1 |
| Tested applications | ELISA |
| Application notes | standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NISCH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NISCH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NISCH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NISCH in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 3.5h |
| Range | 0.16-10 ng/mL |
| Sensitivity | 0.053 ng/mL |
| Sample Types | Tissue homogenates and other biological fluids. |
| Alternative names | IRAS ELISA Kit, Imidazoline Receptor Candidate ELI Read more... |
| Note | For research use only |
