Human NFκB-p65(Nuclear Factor Kappa B p65) Microsample ELISA Kit

• Catalog Number: orb2999004
• Category: Assays and Kits
• Description: Human NFκB-p65(Nuclear Factor Kappa B p65) Microsample ELISA Kit
• UniProt ID: Q04206
• Application notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.053 ng/mL
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Alternative names: NFKB3; p65; REL-A; Nuclear Factor Of Kappa Light P
• Note: For research use only
• Expiration Date: Please enquire.