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Catalog Number | orb775666 |
---|---|
Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Neurotensin(NT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neurotensin(NT). |
Tested applications | ELISA |
Reactivity | Human |
Range | 46.88-3000 pg/mL |
Assay Time | 2.5h |
Concentration | 3000 pg/mL |
UniProt ID | P30990 |
Sensitivity | 14.2 pg/mL |
Alternative names | NTS ELISA Kit, NTS1 ELISA Kit, NmN-125 ELISA Kit, Read more... |
Note | For research use only |
Application notes | standard: 3000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | serum, plasma and other biological fluids |
Expiration Date | Please enquire. |
Human | |
46.88 pg/mL-3000 pg/mL | |
11.72 pg/mL |
Human | |
15.6 pg/ml - 1000pg/ml | |
3.9 pg/ml |