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Catalog Number | orb753149 |
---|---|
Category | Proteins |
Description | NCAM1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 593 amino acids (20-603 a.a) and having a molecular mass of 65.7kDa.NCAM1 is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques. |
Form/Appearance | Sterile filtered colorless solution. |
Buffer/Preservatives | The NCAM1 solution (1mg/ml) contains Phosphate-Buffered Saline (pH 7.4) and 10% glycerol. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Protein Sequence | ADPLQVDIVP SQGEISVGES KFFLCQVAGD AKDKDISWFS PNGEKLTPNQ QRISVVWNDD SSSTLTIYNA NIDDAGIYKC VVTGEDGSES EATVNVKIFQ KLMFKNAPTP QEFREGEDAV IVCDVVSSLP PTIIWKHKGR DVILKKDVRF IVLSNNYLQI RGIKKTDEGT YRCEGRILAR GEINFKDIQV IVNVPPTIQA RQNIVNATAN LGQSVTLVCD AEGFPEPTMS WTKDGEQIEQ EEDDEKYIFS DDSSQLTIKK VDKNDEAEYI CIAENKAGEQ DATIHLKVFA KPKITYVENQ TAMELEEQVT LTCEASGDPI PSITWRTSTR NISSEEKTLD GHMVVRSHAR VSSLTLKSIQ YTDAGEYICT ASNTIGQDSQ SMYLEVQYAP KLQGPVAVYT WEGNQVNITC EVFAYPSATI SWFRDGQLLP SSNYSNIKIY NTPSASYLEV TPDSENDFGN YNCTAVNRIG QESLEFILVQ ADTPSSPSID QVEPYSSTAQ VQFDEPEATG GVPILKYKAE WRAVGEEVWH SKWYDAKEAS MEGIVTIVGL KPETTYAVRL AALNGKGLGE ISAASEFKTQ PVHSPPPHHH HHH |
Application notes | Recombinant & Natural Proteins |
Source | Sf9, Baculovirus cells |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
Alternative names | Neural cell adhesion molecule 1, Neural cell adhes Read more... |
Note | For research use only |
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 77.0 kDa after removal of the signal peptide.The apparent molecular mass of CD56-His is approximately 100-130 kDa due to glycosylation. | |
Mammalian |
Greater than 85% as determined by SDS-PAGE. | |
75.9 kDa | |
E.coli |
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 102.3 kDa after removal of the signal peptide.The apparent molecular mass of CD56-hFc is approximately 130-250 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |