Human Napsin A Aspartic Peptidase (NAPSA) ELISA Kit

• Catalog Number: orb778781
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Napsin A Aspartic Peptidase(NAPSA).
• Tested applications: ELISA
• Reactivity: Human
• Range: 46.88-3000 pg/mL
• Assay Time: 3.5h
• Concentration: 3000 pg/mL
• UniProt ID: O96009
• Sensitivity: 17.38 pg/mL
• Alternative names: KAP ELISA Kit, NAP1 ELISA Kit, ASP4 ELISA Kit, NAP
• Note: For research use only
• Application notes: standard: 3000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NAPSA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NAPSA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NAPSA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NAPSA in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Expiration Date: Please enquire.