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Human N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP) ELISA Kit

Catalog Number: orb778590

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$ 310.00
Catalog Numberorb778590
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with N-Terminal Pro-C-Type Natriuretic Peptide(NT-ProCNP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to N-Terminal Pro-C-Type Natriuretic Peptide(NT-ProCNP).
ReactivityHuman
Concentration1000 pg/mL
Tested applicationsELISA
Application notesstandard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NT-ProCNP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProCNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProCNP in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time2.5h
Range15.63-1000 pg/mL
Sensitivity5.49 pg/mL
Sample TypesSerum, plasma, tissue homogenates and other biological fluids
Alternative namesN-CNP ELISA Kit
NoteFor research use only
Human N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP) ELISA Kit