Human Myosin Light Chain 2, Regulatory, Cardiac (MYL2) ELISA Kit

• Catalog Number: orb776154
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Light Chain 2, Regulatory, Cardiac(MYL2).
• Reactivity: Human
• Concentration: 500 pg/mL
• UniProt ID: P10916
• Tested applications: ELISA
• Application notes: standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MYL2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MYL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MYL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MYL2 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 7.82-500 pg/mL
• Sensitivity: 3.2 pg/mL
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Alternative names: CMH10 ELISA Kit, MLC2 ELISA Kit, Myosin,Light Chai
• Research Area: Cardiovascular Research, Metabolism Research, Musc
• Note: For research use only
• Expiration Date: Please enquire.