Human Myo Inositol Oxygenase (MIOX) ELISA Kit

SKU: orb777236

Description

The Human Myo Inositol Oxygenase (MIOX) ELISA Kit uses a sandwich enzyme immunoassay to quantify Myo Inositol Oxygenase (MIOX) in human tissue homogenates, cell lysates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.057 ng/mL.

Research Area

Metabolism Research; Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MIOX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MIOX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MIOX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MIOX in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.057 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

ALDRL6 ELISA Kit, KSP32 ELISA Kit, RSOR ELISA Kit, Kidney-Specific Protein 32 ELISA Kit, Aldehyde Reductase(Aldose Reductase)Like 6 ELISA Kit, Renal-specific oxidoreductase ELISA Kit, Myo-inositol oxygenase ELISA Kit

Quick Database Links

UniProt

Q9UGB7